Development of a rapid recombinase polymerase amplification assay for detection of Brucella in blood samples
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- 作者：Hang Rena ; 1 ; Mingjuan Yanga ; 1 ; Guoxia Zhangb ; 1 ; Shiwei Liuc ; 1 ; Xinhui Wanga ; Yuehua Kea ; Xinying Dua ; Zhoujia Wanga ; Liuyu Huanga ; Chao Liua ; Zeliang Chena ; zeliangchen@yahoo.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Brucella ; Recombinase polymerase amplification ; Point-of-care diagnosis
- 刊名：Molecular and Cellular Probes
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：30
- 期：2
- 页码：122-124
- 全文大小：790 K
文摘
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A rapid and sensitive Bruce-RPA assay was developed for detection of Brucella.
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The assay could detect as few as 3 copies of Brucella per reaction within 20 min.
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It represents a candidate point-of-care diagnosis assay for human brucellosis.