Aspirin-triggered lipoxin A₄ attenuates lipopolysaccharide induced inflammatory response in primary astrocytes

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• 作者：Chengye Yao ; Dong Yang ; Zhenzhen Wan ; Zhenxing Wang ; Rengang Liu ; Yan Wu ; Shanglong Yao ; Shiying Yuan ; You Shang
• 关键词：Astrocyte ; Lipopolysaccharide ; Aspirin-triggered-lipoxinA4 ; Neuroinflammation
• 刊名：International Immunopharmacology
• 出版年：January, 2014
• 年：2014
• 卷：18
• 期：1
• 页码：85-89
• 全文大小：535 K

文摘

The activation of astrocytes contributes to inflammatory responses underlying brain injury and neurodegenerative diseases. Lipoxins have emerged as mediators of endogenous anti-inflammatory events. However, the involvement of aspirin-triggered-lipoxin A₄ (ATL) in astrocyte-induced neuroinflammatory responses has not been investigated. Here, we examined the anti-inflammatory effects of ATL in the central nervous system using rat astrocyte cultures stimulated with lipopolysaccharide (LPS). We found that pretreatment with ATL exerted potent anti-inflammatory effects by inhibiting LPS-induced production of nitric oxide and prostaglandin E₂. ATL also reduced the expression of cyclooxygenase 2 and inducible nitric oxide synthase mRNA and protein. Furthermore, ATL suppressed the LPS-induced translocation of the NF-魏B p65 subunit to the nucleus and prevented LPS-induced I魏B伪 phosphorylation in a dose-dependent manner. These findings suggest that ATL attenuates neuroinflammation by inhibiting the NF-魏B signal transducer pathway in cultured cortical astrocytes.