Ligustilide prevents the apoptosis effects of tumour necrosis factor-alpha during C2C12 cell differentiation

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• 作者：Ying Shi^a ; ^b ; ^{shiying3646@163.com" class="auth_mail} ; Dongtao Wang^a ; ^b ; ^{95401864@qq.com" class="auth_mail} ; Lu Lu^a ; ^b ; ^{258423864@qq.com" class="auth_mail} ; Yi Yin^a ; ^b ; ^{806982591@qq.com" class="auth_mail} ; Ming Wang^a ; ^b ; ^{66197954@qq.com" class="auth_mail} ; Chengjie Li^a ; ^b ; ^{173584732@qq.com" class="auth_mail} ; Jianxin Diao^b ; ^{393685483@qq.com" class="auth_mail} ; Yanjing Wang^a ; ^{397271626@qq.com" class="auth_mail} ; Lianbo Wei^a ; ^b ; ^{weilianbo@163.com" class="auth_mail}
• 关键词：TNF-伪 ; tumour necrosis factor alpha ; FBS ; foetal bovine serum ; DMEM/F-12 ; Dulbecco's Modified Eagle's Medium F12 Liquid ; MTT ; 3-(4 ; 5-dimethylthiazol-2-yl)-2 ; 5-diphenyl-tetrazo-lium bromide ; DMSO ; dimethyl sulfoxide ; GM ; growth medium ; DM ; differentiation medium ; OD ; optical density ; AI ; apoptosis index ; EB ; ethidium bromide ; bHLH ; helix&ndash ; loop&ndash ; helix ; MRFs ; myogenic regulatory factors
• 刊名：International Immunopharmacology
• 出版年：April, 2014
• 年：2014
• 卷：19
• 期：2
• 页码：358-364
• 全文大小：1176 K

文摘

Ligustilide, the major component of Angelica sinensis, is also thought to be the most potent bioactive constituent of this plant. Ligustilide has been reported to markedly protect neural tissue against apoptosis. However, little is known regarding ligustilide's anti-apoptosis effect in muscle tissue. The aim of the study was to investigate the anti-apoptosis effects of ligustilide on TNF-伪-induced C2C12 cells during differentiation. It was revealed that ligustilide at various concentrations significantly prevented the apoptosis of C2C12 cells incubated in TNF-伪 as assessed by apoptosis index and DNA fragmentation. Moreover, ligustilide-treated groups exhibited a significant increase in the bcl-2/bax ratio, pro-caspase 3 and pro-caspase 8 compared with the TNF-伪-control group in a dose-dependent manner. Meanwhile, ligustilide-treated groups presented a significantly increased level of phosphorylated Akt and suppressed expression of the myogenin protein. Therefore, the findings derived suggested that ligustilide protected C2C12 cells from TNF-伪-induced apoptosis during differentiation by reducing apoptosis and inducing cell proliferation.