Isoflurane decreases proliferation of hippocampal NPCs from developing brain.
Isoflurane increased neuronal differentiation from hippocampal NPCs.
Caspase-3 plays critical roles in isoflurane-induced NPCs exhaustation.
PTEN acted downstream of caspase-3 in isoflurane-induced NPCs exhaustation.
Inhibition of caspase-3 or PTEN attenuates isoflurane-induced NPCs exhaustation.