Correlation between Shiga toxin B-subunit stability and antigen crosspresentation: A mutational analysis

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• 作者：David G. Pina ; Bahne Stechmann ; Valery L. Shnyrov ; Lucien Cabanié ; Nacilla Haicheur ; Eric Tartour ; Ludger Johannes
• 关键词：Shiga toxin B-subunit ; Retrograde transport ; Membrane translocation ; Crosspresentation ; Antigen presenting cell
• 刊名：FEBS Letters
• 出版年：2008
• 出版时间：23 January 2008
• 年：2008
• 卷：582
• 期：2
• 页码：185-189
• 全文大小：919 K

文摘

The homopentameric B-subunit of Shiga toxin (STxB) is used as a tool to deliver antigenic peptides and proteins to the cytosolic compartment of dendritic cells (DCs). In this study, a series of interface mutants of STxB has been constructed. All mutants retained their overall conformation, while a loss in thermal stability was observed. This effect was even more pronounced in trifluoroethanol solutions that mimic the membrane environment. Despite this, all mutants were equally efficient at delivering a model antigenic protein into the MHC class I-restricted antigen presentation pathway of mouse DCs, suggesting that the structural stability of STxB is not a key factor in the membrane translocation process.