Metabolic engineering toward enhanced LC-PUFA biosynthesis in Nannochloropsis oceanica: Overexpression of endogenous 螖12 desaturase driven by stress-inducible promoter leads to enhanced deposition of polyunsaturated fatty acids in TAG
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- 作者：Yuval Kaye ; 1 ; ykaye@carnegiescience.edu" class="auth_mail" title="E-mail the corresponding author ; Omer Grundman1 ; Stefan Leu ; Aliza Zarka ; Boris Zorin ; Shoshana Didi-Cohen ; Inna Khozin-Goldberg ; khozin@bgu.ac.il" class="auth_mail" title="E-mail the corresponding author ; Sammy Boussiba
- 关键词：LC-PUFA ; long-chain polyunsaturated fatty acid ; OA ; oleic acid ; LA ; linoleic acid ; AA ; arachidonic acid ; EPA ; eicosapentaenoic acid ; TAG ; triacylglycerol ; CoA ; coenzyme A ; PC ; phosphatidylcholine ; PE ; phosphatidylethanolamine ; DGTS ; diacylglyceroltrimethylhomoserine ; NoD12 ; Nannochloropsis oceanica microsomal 螖12-desaturase ; LDSP ; lipid droplet surface protein
- 刊名：Algal Research
- 出版年：2015
- 出版时间：September 2015
- 年：2015
- 卷：11
- 期：Complete
- 页码：387-398
- 全文大小：1203 K
文摘
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Functional characterization of Nannochloropsis oceanica microsomal 螖12-desaturase
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Overexpression of endogenous 螖12-desaturase driven by stress-inducible promoter
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Improved deposition of unsaturated fatty acids in TAG of transgene microalgae
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Demonstration of feasibility of LC-PUFA biosynthesis modification in N. oceanica