Autophagy modulators regulate survival motor neuron protein stability in motoneurons
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- 作者：Ambika Periyakaruppiah ; Sandra de la Fuente ; Saravanan Arumugam ; Nú ; ria Bahí ; Ana Garcera1 ; Rosa M. Soler ; 1 ; rosa.soler@cmb.udl.cat" class="auth_mail" title="E-mail the corresponding author
- 关键词：SMA ; Spinal Muscular Atrophy ; MN ; motoneuron ; SMN ; human survival motor neuron protein ; Smn ; murine survival motor neuron protein ; NTF ; neurotrophic factor ; BDNF ; brain-derived neurotrophic factor ; GDNF ; glial cell line-derived neurotrophic factor ; CNTF ; ciliary neurotrophic factor ; CT-1 ; cardiotrophin I ; HGF ; hepatocyte growth factor ; shRNA ; short hairpin RNA ; GFP ; green fluorescent protein ; EV ; empty vector ; WT ; wild type ; mutSMA ; SMA mice mutant ; Calp ; calpain
- 刊名：Experimental Neurology
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：283
- 期：part_PA
- 页码：287-297
- 全文大小：1358 K
文摘
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Autophagosomes are increased in Smn-reduced embryonic and postnatal mouse motoneurons.
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p62/SQSTM1 is increased in cultured mouse Spinal Muscular Atrophy motoneurons.
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The autophagy inhibitor Bafilomycin A1 reduces Smn level in cultured motoneurons.
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Rapamycin treatment causes an increase of autophagy and Smn protein in motoneurons.
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Endogenous calpain knockdown increases Smn protein level in Smn-reduced motoneurons.