Guidelines for the optimal design of miRNA-based shRNAs
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- 作者：Xavier Bofill-De Ros ; Shuo Gu ; shuo.gu@nih.gov" class="auth_mail" title="E-mail the corresponding author
- 关键词：RNAi ; shRNA ; siRNA ; miRNA ; Gene therapy ; RNA structure
- 刊名：Methods
- 出版年：2016
- 出版时间：1 July 2016
- 年：2016
- 卷：103
- 期：Complete
- 页码：157-166
- 全文大小：794 K
文摘
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Overview of the pros and cons of RNA Pol II/III promoters in transcribing shRNA.
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Review of the structural and sequence features involved in miRNA/shRNA maturation.
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Overview on the target sequence selection and off-target effect avoidance.
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Future challenges and potentialities of miRNA-based shRNAs.