Hypochlorous acid turn-on boron dipyrromethene probe based on oxidation of methyl phenyl sulfide

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• 作者：Shi-Rong Liu ; Mani Vedamalai ; Shu-Pao Wu
• 关键词：Hypochlorous acid ; Fluorescent probe ; Boron dipyrromethene ; Bioimaging
• 刊名：Analytica Chimica Acta
• 出版年：2013
• 出版时间：24 October, 2013
• 年：2013
• 卷：800
• 期：Complete
• 页码：71-76
• 全文大小：973 K

文摘

A boron dipyrromethene (BODIPY)-based fluorometric probe, HCS, has been successfully developed for the highly sensitive and selective detection of hypochlorous acid (HOCl). The probe is based on the specific HOCl-promoted oxidation of methyl phenyl sulfide. The reaction is accompanied by a 160-fold increase in the fluorescent quantum yield (from 0.003 to 0.480). The fluorescent turn-on mechanism is accomplished by suppression of photoinduced electron transfer (PET) from the methyl phenyl sulfide group to BODIPY. The fluorescence intensity of the reaction between HOCl and HCS shows a good linearity in the HOCl concentration range 1-10 ¦ÌM. The detection limit is 23.7 nM (S/N = 3). In addition, confocal fluorescence microscopy imaging using RAW264.7 macrophages demonstrates that the HCS probe could be an efficient fluorescent detector for HOCl in living cells.