The mixed ${\text{ClO}}_4^{-}$-acclimated culture can reduce 50–1500 mg L⁻¹${\text{ClO}}_4^{-}$ efficiently.

Perchlorate reduction kinetics was well fitted by Monod equation.

${\text{NO}}_3^{-}$ had a greater inhibition effect for perchlorate reduction than ${\text{SO}}_4^{2-}$.

The optimum temperature, pH and acetate dosage were examined.

Dechloromonas is the dominant bacterium in the acclimated culture.