Highest enzyme loading and enzymatic activity were obtained in PLA2-Fe3O4/SiOx-g-P(GMA).
Residual phosphorus content and free fatty acids content were 9.7 mg/kg and 0.84 g/100 g.
PLA2 can hydrolyze Sn-2 ester bond of phospholipids without acyl migration.
The problem of the high free fatty acid content and low efficiency could be solved.
The magnetic immobilized PLA2 still possessed more than 80% of its initial activity.