Recombinant preparation and functional studies of EspI ATP binding domain from Mycobacterium tuberculosis
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- 作者：Hanyu Chen ; Huilin Wang ; Tao Sun ; Shuangliang Tian ; Donghai Lin ; Chenyun Guo ; guochy78@xmu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：MtEspI415&ndash ; 493 ; Bioinformatics analysis ; Recombinant preparation ; 31P NMR titration
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：123
- 期：Complete
- 页码：51-59
- 全文大小：1341 K
文摘
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The MtEspI ATP binding domain was solubly expressed in Escherichia coli.
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The purity of MtEspI415–493 was 96% after purified by GST- and His-dual-tag.
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Recombinant MtEspI415–493 possessed the ATP binding activity.