Identification of chemical constituents in two traditional Chinese medicine formulae by liquid chromatography-mass spectrometry and off-line nuclear magnetic resonance
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- 作者：Shufang Wang ; wangsf@zju.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Yunxiang Zhu ; Qing Shao ; Yi Wang ; Xiaohui Fan ; Yiyu Cheng
- 关键词：Zhi-Zi-Gan-Cao-Chi-Tang ; Zhi-Zi-Bai-Pi-Tang ; Liquid chromatography&ndash ; mass spectrometry (LC&ndash ; MS) ; Nuclear magnetic resonance (NMR) ; Semi-preparative liquid chromatography
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2016
- 出版时间：5 January 2016
- 年：2016
- 卷：117
- 期：Complete
- 页码：255-265
- 全文大小：2474 K
文摘
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Combining LC–MS with semi-preparative LC and NMR was efficient to identify isomers.
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123 constituents were identified in two TCM formulae by LC–MS and NMR.
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21 constituents without reference standards were prepared by semi-preparative LC.
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Three epimers of known compounds were showed by NMR analysis to be novel compounds.
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Characteristic fragmentation patterns of iridoids and alkaloids were discussed.