We characterized the infection of an EBOV-GP pseudotyped HIV-based vector in human endothelial cells and macrophages.

We identified an aqueous extract of Prunella vulgaris which displayed a potent inhibition of EBOV-GP mediated viral entry.

The inhibitory effect of Chinese herb P. vulgaris (CHPV) is through a directly binding to EBOV-GP.

The presence of CHPV enhances antiviral activity of a monoclonal antibody MAb against EBOV-GP.

This study provides the evidence that CHPV has potential to be developed as a new anti- EBOV agent.