Analysis of aristolochic acid in nine sources of Xixin, a traditional Chinese medicine, by liquid chromatography/atmospheric pressure chemical ionization/tandem mass spectrometry
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- 作者：Jong ; Ting-Ting ; Lee ; Maw-Rong ; Hsiao ; Shun-Sheng ; Hsai ; Jar-Lung ; Wu ; Tian-Shung ; Chiang ; Shu Tuan ; et. al.
- 关键词：Aristolochiaceae ; Aristolochic acid ; Asarum species ; LC/APCI/MS/MS ; Xixin
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2003
- 出版时间：November 24, 2003
- 年：2003
- 卷：33
- 期：4
- 页码：831-837
- 全文大小：392 K
文摘
Aristolochic acid I (AA-I), which is a known nephrotoxin, is found in a commonly used Chinese medicine, Xixin, that originates from nine Asarum species (Aristolochiaceae) found in China. A method has been developed using reversed-phase liquid chromatography coupled with atmospheric pressure chemical ionization (APCI) tandem mass spectrometry under the positive ion detection mode [LC/(+)APCI/MS/MS] to determine the amount of AA-I in Xixin. The limit of detection of AA-I, estimated by monitoring with LC/MS/MS, was at the low μg/l level. By applying this method to methanol extracts of nine Asarum species, the concentrations of AA-I were found to range from 3.3 ng/mg (Asarum sieboldii) to 3376.9 ng/mg (Asarum crispulatum).