A simple and fast HILIC-CAD method was developed for monosaccharides profiling.
Effects of column type, additives, temperature and pH were studied for optimal conditions.
Validation of the method showed good linearity, accuracy, precision and sensitivity.
Successfully applied to monosaccharide analysis of stachyose and EAPW.
First report on the simultaneous and direct determination of the eight sugars by HPLC.