Expression of a vitelline membrane protein, BmVMP23, is repressed by bmo-miR-1a-3p in silkworm, Bombyx mori

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• 作者：Anli Chen ; Dingguo Xia ; Zhiyong Qiu ; Peng Gao ; Shunming Tang ; Xingjia Shen ; Feng Zhu ; Qiaoling Zhao ; ^{qlzhao302@126.com}
• 关键词：BmVMP23 ; 3&prime ; UTR ; MicroRNAs ; Gene expression ; Bombyx mori
• 刊名：FEBS Letters
• 出版年：2013
• 出版时间：2 April, 2013
• 年：2013
• 卷：587
• 期：7
• 页码：970-975
• 全文大小：831 K

文摘

Previous studies have shown that the 3¡ä UTR of BmVMP23 was destroyed by an inserted fragment, and the BmVMP23 expression was downregulated in the lethal egg of ¡°Ming¡± (l-e^m). In this study, we found a miRNA (bmo-miR-1a-3p) that matches the 3¡ä UTR sequence of BmVMP23 perfectly. The relationship between bmo-miR-1a-3p and the BmVMP23 expression was examined by the co-transfection in vitro. The luciferase assay result showed that luc expression was strongly repressed. This result inferred that bmo-miR-1a-3p may downregulate BmVMP23 expression via complementary interaction with the target sites at the 3¡ä UTR.