A possible mechanism for atherosclerosis induced by polycyclic aromatic hydrocarbons
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- 作者：Shunsuke Iwano ; Manabu Nukaya ; Tetsuya Saito ; Fumie Asanuma and Tetsuya Kamataki
- 关键词：PAHs ; LXR ; ABCA1 ; SREBP-1c ; FAS ; SCD ; Quantitative RT-PCR ; Luciferase assay
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2005
- 出版时间：2005
- 年：2005
- 卷：335
- 期：1
- 页码：220-226
- 全文大小：163 K
文摘
Polycyclic aromatic hydrocarbons (PAHs), aryl hydrocarbon receptor (AHR) ligands, induce atherogenesis. Liver X receptor (LXR) α is known to be involved in the control of cholesterol homeostasis. Thus, the purpose of this study was to investigate the effects of 3-methlycholanthrene (MC), one of the PAHs, on LXRα-mediated signal transductions. We found that expression of mRNAs for ATP binding cassette A1, sterol regulatory element binding protein 1c (SREBP-1c), fatty acid synthase, and stearoyl-CoA desaturase was suppressed by treatment of HepG2 cells with MC. A luciferase reporter assay revealed that LXRα- and SREBP-1c-mediated transactivations were inhibited by MC via AHR. Based on these lines of evidence, we propose that down-regulation of the LXRα-regulated genes by PAHs is one of the causes responsible for atherosclerosis induced by PAHs.