Molecular cloning and gene expression of the prox1a

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• 作者：Tomonori Deguchi ; Kazuhiro E. Fujimori ; Takashi Kawasaki ; Hajime Ohgushi ; Shunsuke Yuba
• 关键词：prox1a ; prox1b ; Medaka ; CNS ; Habenula ; Asymmetry
• 刊名：Gene Expression Patterns
• 出版年：2009
• 出版时间：June 2009
• 年：2009
• 卷：9
• 期：5
• 页码：341-347
• 全文大小：908 K

文摘

Prox1 is a prospero-related homeobox gene. Prox1 is expressed in various internal organs and is related to those differentiations. Small fishes such as the zebrafish and the medaka are useful model animals in the clarification of the mechanism of development. The zebrafish prox1 is also identified, and it contributes to clarifying the function of prox1. However, it is necessary to note that many genes are duplicated in teleost fishes. In this study, we identified the orthologs of the mammalian prox1 gene in the medaka. The gene was also duplicated in the medaka, and we named it prox1a and prox1b. In silico analysis from the perspective of synteny indicated that medaka prox1a was similar to the prox1 gene of other vertebrates. Medaka prox1a was expressed in all internal organs that we have examined by RT-PCR. In contrast, medaka prox1b expression was limited to the brain, heart, liver, kidney, thymus, gill, testis, and ovary. This suggests that the two prox1 genes do not have a complementary relationship. In addition, we examined their expression patterns during embryonic development using whole-mount in situ hybridization. The expression pattern of prox1a showed a pattern similar to that of zebrafish prox1. In contrast, medaka prox1b was expressed asymmetrically in part of the central nervous system, especially strongly in the right side of the habenula.