- 作者：Shuqiang Liua ; b ; Zhibin Tana ; Pingting Lia ; Xiaoling Gaoa ; Yuaner Zenga ; Shuling Wanga ; jingelwang@gzucm.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：TCM ; traditional Chinese medicine ; HPLC-ESI-MS ; high performance liquid chromatography-electrospray ionization-mass spectrometry ; SSBR ; saikosaponins of Bupeuri radix ; SSb1 ; saikosaponin b1 ; SSb2 ; saikosaponin b2 ; DiI-HDL ; 1 ; 1&prime ; -Dioctadecyl-3 ; 3 ; 3&prime ; 3&prime ; -tetramethylindocarbocyanine per chlorate (DiI)-labeled human high density lipoprotein ; HFD ; high-fat-diet ; HPLC-MS ; high performance liquid chromatography-mass spectrometry ; HDL-C ; high density lipoprotein cholesterol ; RCT ; reverse
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2016
- 出版时间：20 March 2016
- 年：2016
- 卷：121
- 期：Complete
- 页码：56-62
- 全文大小：1324 K
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In this study, the potential antiatherosclerotic active components in the Radix Bupeuri extract were studied using HepG2Cells biospecific extraction and high performance liquid chromatography-electron spray ionization-mass spectrometry (HPLC-ESI-MS).
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Five compounds in the saikosaponins of Radix Bupeuri (SSRB) were detected as the components selectively combined with HepG2 cells, among these compounds, two potential bioactive compounds, saikosaponin b1 (SSb1) and saikosaponin b2 (SSb2) were identified by comparing with the chromatography of the standard sample and analysis of the structure clearage characterization of mass spectrometry.
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To test whether SSb2 enhanced the selective uptake of lipids from HDL by fluorescence-labeled DiI-HDL uptake after 4 h incubation with HepG2 cells was measured in the presence of indicated concentration of SSb2 or vehicle (0.1% DMSO).
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In conclusion, the application of HepG2 biospecific extraction coupled with HPLC-ESI-MS analysis is a rapid, convenient and reliable method for screening potent active components in TCM and SSb2 may be of value as a novel drug agent for the atherosclerosis.