An ultra-sensitive monoclonal antibody-based enzyme-linked immunosobent assay for dibutyl phthalate in human urinary
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- 作者：Lifang Zhoua ; Yajing Leib ; Dai Zhanga ; Shabbir Ahmeda ; Shuqing Chena ; chenshuqing@zju.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Dibutyl phthalate ; Monoclonal antibody ; Urinary samples ; Internal exposure
- 刊名：Science of the Total Environment
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：541
- 期：Complete
- 页码：570-578
- 全文大小：1064 K
文摘
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DBP complete antigens (DBP–BSA and DBP–OVA) were designed and synthesized novelty.
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The development of DBP monoclonal antibody with no cross-reactivity, low LOD (0.06 ng mL− 1) and IC50 (7.34 ng mL− 1)
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Optimization and evaluation of indirect complete competitive enzyme immunoassay
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Application of the method in detecting DBP of internal exposure among Chinese human urinary
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Subjects were randomly recruited and system classified by ages, genders and educations.