Sensitive determination of four tetracycline antibiotics in pig plasma by field-amplified sample stacking open-tubular capillary electrochromatography with dimethylethanolamine aminated polychloromethyl styrene nano-latex coated capillary column

详细信息    查看全文

• 作者：Yaxiao Guo ; Lei Meng ; Yanhao Zhang ; Wei Tang ; Wenfen Zhang ; Yan Xia ; Fuguo Ban ; Ningpeng Wu ; Shusheng Zhang
• 关键词：Tetracycline antibiotics ; Plasma ; Open-tubular capillary electrochromatography ; Field-amplified sample stacking ; Dimethylethanolamine aminated polychloromethyl styrene ; Nano-latex
• 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
• 出版年：30 December, 2013
• 年：2013
• 卷：942-943
• 期：Complete
• 页码：151-157
• 全文大小：1194 K

文摘

This paper described the preparation and application of a new dimethylethanolamine aminated polychloromethyl styrene nano-latex (DMEAPL) coated capillary column (ccc-DMEAPL) in the determination of four tetracycline antibiotics (TCA) including tetracycline (TC), oxytetracycline (OTC), doxycycline (DC) and chlorotetracycline (CTC) in pig plasma. The ccc-DMEAPL column was characterized with steady EOF values of ca. 1.5-5.2 脳 10^鈭? cm²/V s at pH 1.8-6.3. The optimized conditions for field-amplified sample stacking open-tubular capillary electrochromatography (FASS-OT-CEC) were as following: background electrolyte, 10 mmol/L Na₂HPO₄ + 15 mmol/L citric acid (pH 3.2); ccc-DMEAPL, 50 渭m i.d. 脳 50 cm (effective length 41.5 cm), separation voltage, 18 kV; column temperature, 25 掳C; UV detection wavelength, 270 nm; water-plug injection: 30 mbar 脳 10 s; sample electrokinetic injection, 10 kV 脳 20 s. The four TCA were extracted with the solution of 10 mmol/L Na₂HPO₄ + 15 mmol/L citric acid + 4 g/L EDTA-2Na (pH 3.2). The FASS-OT-CEC method was validated in terms of linearity, sensitivity, selectivity, precision and accuracy. The LODs ranged from 3 to 7 ng/mL, the recoveries for the four TCA were all more than 80%. The developed method was successfully applied for the determination of TCs in the actual pig plasma samples.