Molecular analysis of surface glycoprotein multigene family TrGP

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• 作者：C.P. Peñ ; a ; N. L ; er ; E. Rodrí ; guez ; G. Crisante ; N. Añ ; ez ; J.L. Ramí ; rez ; M.A. Chiurillo
• 关键词：Trypanosoma rangeli ; gp85/trans-sialidase ; Multigenic family ; TrGP
• 刊名：Acta Tropica
• 出版年：2009
• 出版时间：September 2009
• 年：2009
• 卷：111
• 期：3
• 页码：255-262
• 全文大小：1263 K

文摘

Trypanosoma rangeli, a non-pathogenic hemoflagelate that in Central and South America infects humans, shares with Trypanosoma cruzi reservoirs and triatomine vectors, as well as geographical distribution. Recently, we have described in T. rangeli a truncated gene copy belonging to the group II of the trans-sialidase superfamily (TrGP). This superfamily, collectively known in T. cruzi as gp85/TS, includes members that are involved in host cell invasion and infectivity. To confirm the presence of this superfamily in the genome of T. rangeli and obtain a better knowledge of its characteristics, we designed a PCR and RT-PCR cloning strategy to allow sequence analysis of both genomic and transcribed copies. We identified two full-length copies of TrGP, some pseudogenes, and N- and C-terminal sequences of several genes. We also analyzed the expression and cellular localization of these proteins in epimastigote forms of a Venezuelan T. rangeli isolate using polyclonal antibodies made against a recombinant peptide from the N-terminal region of a TrGP member. We confirmed that TrGP is a multigenic family that shares many features with T. cruzi gp85/TS, including the telomeric location of some of its members, and by immunofluorescence analysis that its location is at the surface of the parasite.