A systematic analysis of acceptor specificity and reaction kinetics of five human α(2,3)sialyltransferases: Product inhibition studies illustrate reaction mechanism for ST3Gal-I
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- 作者：Rohitesh Guptaa ; rohitesh.gupta@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Khushi L. Mattab ; Sriram Neelameghama ; c ; neel@buffalo.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Sialyltransferase ; Michaelis&ndash ; Menten ; Product inhibition ; Mass spectrometry
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：469
- 期：3
- 页码：606-612
- 全文大小：1064 K
文摘
- 10">
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10">KM for five Human ST3Gals is reported towards Type-I, Type-II & Type-III acceptors.
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15">LC-MS simultaneously quantifies CMP-Neu5Ac & Glycans in a sialylation reaction.
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Efficient Core2 sialylation indicates co-operativitiy between ST3Gal-I & C2GnT1.
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ST3Gal-I inhibition study proposes iso- or random-sequential bi-bi mechanism.