Design and analysis of a squamous cell carcinoma in?vitro model system

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• 作者：Eva Brauchle ; Hannah Johannsen ; Samantha Nolan ; Sibylle Thude ; Katja Schenke-Layl
• 关键词：Co-culture ; Epithelium ; In vitro test ; Organ culture ; Keratinocyte
• 刊名：Biomaterials
• 出版年：2013
• 出版时间：October, 2013
• 年：2013
• 卷：34
• 期：30
• 页码：7401-7407
• 全文大小：2571 K

文摘

Tissue-engineered skin equivalents based on primary isolated fibroblasts and keratinocytes have been shown to be useful tools for functional in?vitro tests, including toxicological screenings and drug development. In this study, a commercially available squamous cell carcinoma (SCC) cell line SCC-25 was introduced into epidermal and full-thickness skin equivalents to generate human-based disease-in-a-dish model systems. Interestingly, when cultured either in the epidermis or dermis of full-thickness skin equivalents, SCC-25 cells formed hyper-keratinized tumor cell nests, a phenomenon that is frequently seen in the skin of patients afflicted with SCC. Raman spectroscopy was employed for the label-free cell phenotype characterization within the engineered skin equivalents and revealed the presence of differential protein patterns in keratinocytes and SCC-25 cells. To conclude, the here presented SSC disease-in-a-dish approaches offer the unique opportunity to model SSC in human skin in?vitro, which will allow further insight into SSC disease progression, and the development of therapeutic strategies.