Anti-abortion and Fertility Vaccine Potential of Defined Double Deletion (螖aroA螖htrA) Mutant (S30) of Salmonella Abortusequi in Equids

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• 作者：Mudit Chandra ; PhD^a ; ^b ; ^{drmuditchandra@rediffmail.com" class="auth_mail} ; Bhoj Raj Singh ; PhD^a ; ^c ; Nagarajan Babu ; MVSc^a ; ^d ; Ravi Kant Agarwal ; PhD^a ; ^e ; Mahtab Z. Siddiqui ; PhD^a ; ^f
• 关键词：Oral vaccine ; Equine ; Salmonella Abortusequi ; Deletion mutant
• 刊名：Journal of Equine Veterinary Science
• 出版年：June 2014
• 年：2014
• 卷：34
• 期：6
• 页码：765-773
• 全文大小：538 K

文摘

The present study on defined double deletion (螖aroA螖htrA) mutant (S30) of Salmonella enterica serovar Abortusequi evaluated it for safety, immunogenicity, and efficacy as a vaccine candidate in equids. The candidate strain was found safe in equids (foals, male and female horses and donkeys, and pregnant and nonpregnant mares) and induced good humoral and cell-mediated immunity on administration through oral route. The strain was not excreted in feces of vaccinated animals. The vaccine candidate administered orally (1 × 10¹¹ cfu per animal) protected mares even after 180 days of inoculation against abortion on challenge with wild-type S. Abortusequi strain whereas in nonvaccinated control, all mares aborted. The vaccination in infertile mares resulted in regaining of fertility in 67%–80% thoroughbred mares at two different breeding farms. Further, the humoral immunity was transferred to foals from vaccinated mothers through colostrum, but no placental transfer was evident. Thus, the vaccine under study may be recommended for use in equids to control S. Abortusequi infection–associated abortions and also to enhance fertility of temporarily infertile mares in endemic areas.