Neuronal network properties of human teratocarcinoma cell line-derived neurons

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• 作者：Gö ; rtz ; Philipp ; Fleischer ; Wiebke ; Rosenbaum ; Claudia ; Otto ; Frauke ; Siebler ; Mario
• 关键词：Development and regeneration ; Cell differentiation and migration ; MEA ; Multielectrode array ; Grafting ; Cell culture ; Neuronal network ; Neurochip
• 刊名：Brain Research
• 出版年：2004
• 出版时间：August 20, 2004
• 年：2004
• 卷：1018
• 期：1
• 页码：18-25
• 全文大小：445 K

文摘

Understanding the structural and functional development of neurons in networks has a high impact to estimate the potentials for restorative therapies. Neurons derived from the human NT2 cell line (hNT) formed networks with a clustered neuritic architecture in vitro, whereas primary dissociated embryonic rat cortical neurons (Cx) displayed a more homogenous cell assembly. Spontaneous spikes of both cell types were recorded on microelectrode arrays within 2 weeks after seeding, but hNT showed a mostly uncorrelated firing pattern in contrast to Cx with highly synchronized bursting. hNT neurons were less sensitive to TTX (IC₅₀