Proteome analysis of alfalfa roots in response to water deficit stress

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• 作者：Md Atikur Rahman^a ; ^{atikgnu@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Kim Yong-Goo^a ; Alam Iftekhar^a ; ^c ; Gong-she LIU^b ; Lee Hyoshin^d ; Lee Jeung Joo^e ; Lee Byung-Hyun^a ; ^{hyun@gnu.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：alfalfa ; proteome ; root ; water deficit stress
• 刊名：Journal of Integrative Agriculture
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：15
• 期：6
• 页码：1275-1285
• 全文大小：1463 K

文摘

To evaluate the response of alfalfa to water deficit (WD) stress, WD-induced candidates were investigated through a proteomic approach. Alfalfa seedlings were exposed to WD stress for 12 and 15 days respectively, followed by 3 days re-watering. Water deficit increased H₂O₂ content, lipid peroxidation, DPPH (1,1-diphenyl-2-picrylhydrazyl)-radical scavenging activity, and the free proline level in alfalfa roots. Root proteins were extracted and separated by two-dimentional polyacrylamide gel electrophoresis (2-DE). A total of 49 WD-responsive proteins were identified in alfalfa roots; 25 proteins were reproducibly found to be up-regulated and 24 were down-regulated. Two proteins, namely cytosolic ascorbate peroxidase (APx2) and putative F-box protein were newly detected on 2-DE maps of WD-treated plants. We identified several proteins including agamous-like 65, albumin b-32, inward rectifying potassium channel, and auxin-independent growth promoter. The identified proteins are involved in a variety of cellular functions including calcium signaling, abacisic acid (ABA) biosynthesis, reactive oxygen species (ROS) regulation, transcription/ translation, antioxidant/detoxification/stress defense, energy metabolism, signal transduction, and storage. These results indicate the potential candidates were responsible for adaptive response in alfalfa roots.