MicroRNA expression analysis of rosette and folding leaves in Chinese cabbage using high-throughput Solexa sequencing

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• 作者：Fengde Wang ; Huayin Li ; Yihui Zhang ; Jingjuan Li ; Libin Li ; Lifeng Liu ; Lihua Wang ; Cuihua Wang ; Jianwei Gao
• 关键词：BcpLH ; Brassica campestris ssp. pekinensis leafy heads ; HD-ZIP III ; Homeodomain-leucine zipper protein III ; GRF ; Growth-regulation factor ; TCP ; Teosinte branched1/Cycloidia/PCF ; AP2 ; APETALA2 ; AGL ; Agamous-like ; MYB ; Myeloblastosis ; bZIP ; Basic region/leucine zipper motif ; ARF ; Auxin response factors ; AUX/IAA ; Auxin/indole-3-acetic acid ; GH3 ; Gretchen hagen3 ; PIN ; PIN-formed ; CaM ; Calmodulin ; CaLB ; Calcium-dependent lipid binding ; LBD ; Lateral organ boundaries domain ; AFB3 ; Auxin signaling F-box
• 刊名：Gene
• 出版年：15 December, 2013
• 年：2013
• 卷：532
• 期：2
• 页码：222-229
• 全文大小：629 K

文摘

In this study, a global analysis of miRNA expression from rosette leaves (RLs) and folding leaves (FLs) of Chinese cabbage (Brassica rapa L. ssp. pekinensis) was conducted using high-throughput Solexa sequencing. In total, over 12 million clean reads were obtained from each library. Sequence analysis identified 64 conserved miRNA families in each leaf type and 104 and 95 novel miRNAs from RLs and FLs, respectively. Among these, 61 conserved miRNAs and 61 novel miRNAs were detected in both types of leaves. Furthermore, six conserved and 21 novel miRNAs were differentially expressed between the two libraries. Target gene annotation suggested that these differentially expressed miRNAs targeted transcription factors, F-box proteins, auxin and Ca^2 + signaling pathway proteins, protein kinases and other proteins that may function in governing leafy head formation. This study advanced our understanding of the important roles of miRNAs in regulating leafy head development in Chinese cabbage.