Standardized bovine colostrum derivative impedes development of type 1 diabetes in rodents

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• 作者：Ivana Nikolic^a ; Ivana Stojanovic^a ; Milica Vujicic^a ; Paolo Fagone^b ; Katia Mangano^b ; Stanislava Stosic-Grujicic^a ; Ferdinando Nicoletti^b ; Tamara Saksida ; PhD^a ; ^{cvjetica@ibiss.bg.ac.rs}
• 关键词：DP-BB ; diabetes prone-bio breeding ; IGF ; insulin-like growth factor ; MLDS ; multiple low doses of streptozotocin ; MLN ; mesenteric lymph nodes ; NOD ; non-obese diabetic ; SBCD ; standardized bovine colostrum derivative ; T1D ; type 1 diabetes ; TGF-β ; transforming growth factor-β ; Th ; T helper ; TNF ; tumor necrosis factor
• 刊名：Immunobiology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：222
• 期：2
• 页码：272-279
• 全文大小：1182 K
• 卷排序：222

文摘

Bovine colostrum is a rich source of nutrients and immunologically active components that play a role in conveying passive immunity to the offspring, protection and maturation of new-born’s gastrointestinal tract. Colostrum has exerted positive effects in diseases affecting gastrointestinal tract, as well as type 2 diabetes (T2D). However, health-promoting effects in type 1 diabetes have not been reported. The aim of this study was to investigate therapeutic value of oral administration of standardized bovine colostrum derivative (SBCD) in three models of type 1 diabetes (T1D): spontaneously developed T1D in NOD mice and BB-DP rats, and in chemically induced T1D in C57BL/6 mice with multiple low doses of streptozotocin (MLDS). SBCD was administered per os and the disease development was evaluated by weekly measurement of blood glucose and by histological analyses of the pancreas. SBCD administration prevented diabetes development in all three models, as indicated by euglicaemia. Ex vivo analysis of cytokine expression and production in the spleen and mesenteric lymph nodes (MLN) in MLDS challenged mice revealed a strong modulation of the immune response. In the MLN cells SBCD disrupted harmful Th17 response induced by MLDS. Expression of Th1 signature cytokine IFN-γ was down-regulated in MLN cells of SBCD-treated mice, while IL-4 secretion (Th2 cytokine) was up-regulated in comparison to diabetic group. Modulation of the immune response seen in the MLN protruded to the spleen, giving overall less infiltration of immune cells to the pancreas. SBCD acted on immune cells and halted (auto) aggression towards pancreatic beta cells. Moreover, SBCD induced beta cell proliferation. Hence, this derivative could be tested in diabetes and other similar diseases with aberrant immune response.