Pulse radiolysis and spectrophotometric studies on the binding of organic cations with heparin

详细信息    查看全文

• 作者：Ma艂gorzata Jakubowska ; Jan Adamus ; Jerzy G臋bicki ; Andrzej Marcinek ; Adam Sikora ; ^{adam.sikora@p.lodz.pl" class="auth_mail}
• 关键词：bis(MNA+) ; 1 ; 3-bis(1-methylnicotinamide)propane ; (CH3)3COH ; tert-butanol ; COX-2 ; cyclooxygenase 2 ; eaq ; solvated electron ; GAG壮s ; glycosaminoglycans ; H ; hydrogen atom ; H2O2 ; hydrogen peroxide ; Hep ; heparin ; KSCN ; potassium thiocyanate ; MB ; methylene blue ; MNA+ ; 1-methylnicotinamide ; N2O ; dinitrogen monoxide ; OH ; hydroxyl radical ; PGI2 ; prostacyclin
• 刊名：Radiation Physics and Chemistry
• 出版年：June, 2014
• 年：2014
• 卷：99
• 期：Complete
• 页码：6-11
• 全文大小：779 K

文摘

Here we present the spectroscopic and pulse radiolysis studies of the interactions of heparin and some organic cations:methylene blue (MB), 1-methylnicotinamide (MNA⁺), and its dimer 1,3-bis(1-methylnicotinamide)propane (bis(MNA⁺)).

The interaction between heparin and some cationic dyes (e.g. methylene blue) in aqueous solution leads to the changes in the absorption spectra of those dyes. This effect, called metachromasia, has been successfully used to study the interactions of a number of cationic dyes with heparin and other glycosaminoglycans.

Using methylene blue, as a model compound, we have shown that the changes in the rate constant for the reaction of solvated electrons with cationic dye in the presence of heparin (pulse radiolysis) are well correlated with the accompanying changes in the absorption spectra (metachromasia). The same mathematical formalism was applied to absorption and rate constants changes. Using such formalism, we have determined the total number of binding sites per heparin molecule and the equilibrium constants for MNA⁺ and bis(MNA⁺).