Embryotoxicity of cobalt ferrite and gold nanoparticles: A first in vitro approach

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• 作者：Claudia Di Guglielmo ; David Ramos Ló ; pez ; Joaquí ; n De Lapuente ; Joan Maria Llobet Mallafre ; Miquel Borrà ; s Suà ; rez
• 关键词：Mouse embryonic stem cells test ; Teratology ; Embryotoxicity ; Gold nanoparticles ; Cobalt ferrite nanoparticles ; Cytotoxicity ; Differentiation
• 刊名：Reproductive Toxicology
• 出版年：2010
• 出版时间：September 2010
• 年：2010
• 卷：30
• 期：2
• 页码：271-276
• 全文大小：460 K

文摘

Nanoparticles (NPs) are emerging as promising biomedical tools thanks to their peculiar characteristics. Our purpose was to investigate the embryotoxicity of cobalt ferrite and gold NPs through the Embryonic Stem Cell Test (EST). The EST is an in vitro standard assay, which permits to classify substances as strongly, weakly or non-embryotoxic. Due to the particular physical–chemical nature of nanoparticles, we introduced a modification to the standard protocol exposing the Embryonic Stem Cells (ES-D3) to nanoparticles only during the first 5 days of the assay. Moreover, we proposed a method to discriminate and compare the embryotoxicity of the substances within the weakly embryotoxic range. Our ID₅₀ results permit to classify cobalt ferrite nanoparticles coated with gold and silanes as non-embryotoxic. The remaining nanoparticles have been classified as weakly embryotoxic in this decreasing order: gold salt (HAuCl₄·3H₂O) > cobalt ferrite salt (CoFe₂O₄) > cobalt ferrite nanoparticles coated with silanes (Si–CoFe) > gold nanoparticles coated with hyaluronic acid (HA–Au).