Functional analysis of a weak viral RNA silencing suppressor using two GFP variants as silencing inducers

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• 作者：Krin S. Mann¹ ; Ralf G. Dietzgen ; ^{r.dietzgen@uq.edu.au}
• 关键词：Cytorhabdovirus ; Phosphoprotein ; GFP silencing ; RNA silencing suppression ; N. benthamiana 16c ; Green fluorescent protein
• 刊名：Journal of Virological Methods
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：239
• 期：Complete
• 页码：50-57
• 全文大小：2090 K
• 卷排序：239

文摘

GFP variants induce local and systemic RNA silencing at different rates in plants. eGFP is best for identification of weak RNA silencing suppressors. Slower GFP silencing induction allows discovery of weaker silencing suppressors. LNYV P can suppress systemic silencing when challenged with eGFP but not mGFP5-ER. LNYV P suppresses silencing induced by potent GFP-dsRNA in a dose-dependent manner.