Purification, characterization, and cDNA cloning of a matrix metalloproteinase from the skeletal muscle of silver carp (Hypophthalmichthys molitrix) with collagen degradation activity
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- 作者：Jiulin Wua ; wujiul590@126.com" class="auth_mail" title="E-mail the corresponding author ; Shuang Wanga ; Xinyu Suna ; Minjie Caob ; Mingmao Chena ; Jiabin Wanga ; Qiqing Zhanga ; zhangqiq@126.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Matrix metalloproteinase ; Silver carp ; Characterization ; Collagen ; Postmortem tenderization
- 刊名：Process Biochemistry
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：51
- 期：7
- 页码：854-864
- 全文大小：4905 K
文摘
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A matrix metalloproteinase (MMP) was purified from silver carp muscle.
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The purified proteinase was MMP-2 based on its characteristics and peptide mass fingerprinting (PMF) analysis.
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The full length of the complementary DNA (cDNA) sequence encoding the purified proteinase was cloned.
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The purified proteinase was possibly involved in the postmortem degradation of fish muscle.