EUS-guided Nd:YAG laser ablation of normal pancreatic tissue: a pilot study in a pig model

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• 作者：Francesco Di Matteo ; Margareth Martino ; Roberta Rea ; Monica P ; olfi ; Carla Rabitti ; Grazia Maria Paola Masselli ; Sergio Silvestri ; Claudio Maurizio Pacella ; Enrico Papini ; Francesco Panzera ; Sergio Val
• 刊名：Gastrointestinal Endoscopy
• 出版年：2010
• 出版时间：August 2010
• 年：2010
• 卷：72
• 期：2
• 页码：358-363
• 全文大小：1817 K

文摘

Background

Laser ablation with a neodymium:yttrium aluminum garnet (Nd:YAG) laser can achieve a high rate of complete tissue necrosis and has been applied as a minimally invasive, palliative option in hepatocellular carcinoma, liver metastasis in colorectal cancer, and malignant thyroid nodules.

Objective

To assess the in vivo feasibility of EUS-guided laser ablation with an Nd:YAG laser of normal pancreatic tissue of a porcine model.

Design

Prospective investigation.

Setting

Hospital animal laboratory.

Subjects

Eight pigs.

Interventions

EUS-guided puncture of the pancreatic tail with a laser-beam fiber. An Nd:YAG laser (1.064 nm) was used, with an output power of 2 and 3 W and a total delivered energy of 500 and 1000 J in continuous mode.

Main Outcome Measurements

The 24-hour follow-up of the pigs was focused on clinical and laboratory aspects. Results of histological studies of the pancreas were obtained 24 hours after the procedure on necroscopy tissue.

Results

There were no technical limitations to the performance of the procedure. Tissue necrosis, localized in the pancreatic parenchyma, was observed in all animals on histological examination. The volume of ablation tissue ranged from a mean of 314 mm³ to 483 mm³. The ablation area ranged from a mean of 49 mm² to 80 mm². No major postprocedure complications were recorded, and all the pigs survived at 24 hours.

Limitation

Animal study.

Conclusions

EUS-guided laser ablation of the pancreas with an Nd:YAG laser is feasible in a porcine model.