In vitro myelotoxicity of environmental contaminants
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- 作者：Gribaldo ; Laura ; Casati ; Silvia ; Figliuzzi ; Lucia ; Marafante ; Erminio
- 关键词：Bone marrow culture ; CFU-GM assay ; Environmental toxins ; Haematotoxicity ; Myelotoxicity ; SR-4987 cells
- 刊名：Environmental Toxicology and Pharmacology
- 出版年：1998
- 出版时间：October, 1998
- 年：1998
- 卷：6
- 期：2
- 页码：135-141
- 全文大小：151 K
文摘
Myelotoxicity of pesticides and algal toxins was detected in vitro by using the granulocyte-macrophage colony forming unit assay (CFU-GM), and the MTT test with SR-4987 cells, an established stromal cell line derived from a long term murine bone marrow culture, which may represent a suitable in vitro model for studying haematotoxicity. Comparison of the IC50s and NOELs obtained with the CFU-GM assay and those determined by testing the established stromal cells in the MTT cytotoxicity test indicate that inhibition of the proliferation of SR-4987 stromal cells is a sensitive in vitro endpoint for measuring myelotoxicity. It is suggested that this assay could be used as rapid and easy screening test for determining the haematotoxicity of environmental toxins. A comparison with results obtained with the MTT test on a non-differentiated cell line, 3T3-L1, was carried out to distinguish between non-specific interference with cell proliferation and specific toxicity on haemopoietic cells.