Characterization of integrons and associated gene cassettes in Acinetobacter baumannii strains isolated from intensive care unit in Tehran, Iran

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• 作者：Hossein Goudarzi¹ ; Mehdi Azad² ; Sima Sadat Seyedjavadi³ ; Hadi Azimi⁴ ; Alireza Salimi Chirani¹ ; Vahid Fallah Omrani⁵ ; Mehdi Goudarzi¹ ; ^{gudarzim@yahoo.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Acinetobacter baumannii ; Multidrug resistance ; Integron ; Intensive care unit
• 刊名：Journal of Acute Disease
• 出版年：2016
• 出版时间：1 September 2016
• 年：2016
• 卷：5
• 期：5
• 页码：386-392
• 全文大小：329 K

文摘

To determine the antimicrobial susceptibility patterns, the frequency of integrons and associated gene cassettes in Acinetobacter baumannii (A. baumannii) strains isolated from selected hospital intensive care units.

Methods

During a ten-month period, 120 A. baumannii isolates were studied. The resistance rates to different classes of antimicrobial agents were determined. PCR was used to detect different types of integrons and associated gene cassettes.

Results

The resistance rates to the majority of antibiotics tested were found to be between 39.3% and 99.1%. No isolate was observed to be resistant to colistin and polymyxin B. The rate of extensive drug-resistance among these clinical isolates was 62.5%. The prevalence of class 1 and 2 integrons was found to be 74.1% and 12.5%, respectively. Seven different gene cassettes (ampC, aacA4-catB8, ISAba1-bla_OXA-23-GES-14, aadA2-cm1A6-GES-14-qacF, VIM-25-GES-24-qacF, dfrA5-ISAba1-bla_OXA-51-bla_OXA-40 and aadA2-GES-11-IMP-1) were observed in Class 1 integron-carrying strains. Three gene cassettes (IMP-4, VIM-2-VEB-aacA4 and dfrA2-sat-2-aadA4) were detected in class 2 integron-bearing A. baumannii strains.

Conclusions

A high prevalence of integron was described among multidrug resistant A. baumannii in the hospital. The findings highlighted the need for continuous surveillance in order to prevent dissemination of multidrug resistance among A. baumannii strains in Iran.