Similar effects of resistin and high glucose on P-selectin and fractalkine expression and monocyte adhesion in human endothelial cells

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• 作者：Ileana M ; uteanu ; Monica Pirvulescu ; Ana Maria Gan ; Daniela Stan ; Viorel Simion ; Elena Dragomir ; Manuela Calin ; Adrian Manea ; Maya Simionescu
• 关键词：Resistin ; High glucose ; P-selectin ; Fractalkine ; Monocyte adhesion
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2010
• 出版时间：15 January 2010
• 年：2010
• 卷：391
• 期：3
• 页码：1443-1448
• 全文大小：348 K

文摘

Resistin and high glucose (HG) are concomitantly present at elevated concentration in diabetic’s plasma; both are pro-inflammatory agents acting on vascular cells by mechanisms that are not fully understood. We questioned whether resistin and HG affect the expression of major adhesion molecules, P-selectin and fractalkine in human endothelial cells (HEC). The results showed that in HEC (i) resistin increased P-selectin expression; (ii) HG up-regulated Fk expression; (iii) P-selectin and fractalkine were functional increasing monocyte adhesion to activated cells. Co-stimulation with resistin and HG increased P-selectin and fractalkine mRNA and protein and induced monocyte adhesion, generated an increase in NADPH oxidase activity and of the intracellular reactive oxygen species and activated the NF-kB and AP-1 transcription factors at similar values as those of each activator. In conclusion in HEC, resistin and HG induce the up-regulation of P-selectin and fractalkine and the ensuing increased monocyte adhesion by a mechanism involving oxidative stress and NF-kB and AP-1 activation.