Complex formation of rutin and quercetin with copper alters the mode of inhibition of Ribonuclease A

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• 作者：Debi Ranjan  ; Tripathy ; Atanu  ; Singha Roy ; Swagata  ; Dasgupta  ; ;   ; ^{swagata@iitkgp.ac.in} ;   ; ^{swagata_dasgupta@hotmail.com}
• 关键词：Ribonuclease A ; Quercetin ; Rutin ; Copper complexes ; Inhibition
• 刊名：FEBS Letters
• 出版年：2011
• 出版时间：20 October 2011
• 年：2011
• 卷：585
• 期：20
• 页码：3270-3276
• 全文大小：567 K

文摘

Rutin and quercetin, both minor components of green tea and their Cu(II) complexes interact with Ribonuclease A (RNase A) in a novel way. The effects of rutin, quercetin and their copper complexes on the catalytic activity of the protein were investigated. Rutin shows an enhancement in the ribonucleolytic activity whereas the copper complexes and quercetin behave as non-competitive type inhibitors with K_i values in the ¦ÌM range. The secondary structural changes of RNase A in presence of the ligands were measured by circular dichroism and Fourier transform infrared spectroscopy. The binding parameters were obtained using a fluorescence quenching analysis.