Immobilization of cholesterol esterase and cholesterol oxidase onto sol–gel films for application to cholesterol biosensor
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- 作者：Suman Singh ; Rahul Singhal ; B.D. Malhotra
- 关键词：Tetraethylorthosilicate (TEOS) sol– ; gel ; Cholesterol oxidase ; Cholesterol esterase ; Cholesterol oleate ; Encapsulation ; Cholesterol biosensor
- 刊名：Analytica Chimica Acta
- 出版年：2007
- 出版时间：23 January 2007
- 年：2007
- 卷：582
- 期：2
- 页码：335-343
- 全文大小：637 K
文摘
Cholesterol oxidase (ChOx) and cholesterol esterase (ChEt) have been covalently immobilized onto tetraethylorthosilicate (TEOS) sol–gel films. The tetraethylorthosilicate sol–gel/ChEt/ChOx enzyme films thus prepared have been characterized using scanning electron microscopic (SEM), UV–vis spectroscopic, Fourier-transform-infrared (FTIR) spectroscopic and amperometric techniques, respectively. The results of photometric measurements carried out on tetraethylorthosilicate sol–gel/ChEt/ChOx reveal thermal stability up to 55 °C, response time as 180 s, linearity up to 780 mg dL−1 (12 mM), shelf life of 1 month, detection limit of 12 mg dL−1 and sensitivity as 5.4 × 10−5 Abs. mg−1 dL−1.