Surface and bioproperties of nanocrystalline diamond/amorphous carbon nanocomposite films

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• 作者：W. Kulisch ; C. Popov ; S. Bliznakov ; G. Ceccone ; D. Gillil ; L. Sirghi ; F. Rossi
• 关键词：Nanocrystalline diamond films ; Surface characterization ; XPS ; TOF-SIMS ; Bioproperties
• 刊名：Thin Solid Films
• 出版年：2007
• 出版时间：14 September 2007
• 年：2007
• 卷：515
• 期：23
• 页码：8407-8411
• 全文大小：410 K

文摘

Nanocrystalline diamond/amorphous carbon (NCD/a-C) nanocomposite films have been deposited by microwave plasma chemical vapour deposition from CH₄/N₂ mixtures. In order to investigate their suitability as templates for the immobilization of biomolecules, e.g. for applications in biosensors, four differently prepared surfaces, namely as-grown, hydrogen plasma treated, oxygen plasma treated, and chemically treated with aqua regia, have been thoroughly characterized by methods such as XPS, TOF-SIMS, AFM, and contact angle measurements. In addition, in order to investigate the affinity of these surface to non-specific bonding of biomolecules, they have been exposed to bovine serum albumin (BSA). It turned out that already the as-grown surface is hydrogen terminated; the degree of the termination is even slightly improved by the hydrogen plasma treatment. Reaction with aqua regia, on the other hand, led to a partial destruction of the H-termination. The oxygen plasma treatment, finally, causes a termination by O and OH, rather than by carboxylic acid groups. In addition, an increase of sp² bonded carbon is observed. All surfaces were found to be susceptible to attachment of BSA proteins, but the coverage of the hydrogen terminated was lower than that of the O-terminated film. The highest BSA concentrations were found for the aqua regia sample where the H-termination has been removed partially. Finally, our results show that even minor surface contaminations have a great influence on the BSA coverage.