Xenobiotic metabolism of plant secondary compounds in the English grain aphid, Sitobion avenae (F.) (Hemiptera: Aphididae)

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• 作者：Maoxin Zhang ; Tingting Fang ; Guilin Pu ; Xiaoqin Sun ; Xuguo Zhou ; Qingnian Cai
• 关键词：Secondary plant compounds ; Sitobion avenae ; Phase I and II detoxification enzymes ; Xenobiotic metabolism ; Plant defense
• 刊名：Pesticide Biochemistry and Physiology
• 出版年：2013
• 出版时间：September, 2013
• 年：2013
• 卷：107
• 期：1
• 页码：44-49
• 全文大小：989 K

文摘

Plant secondary compounds have been documented to be deleterious to insects and other herbivores in diverse ways. In this study, the effect of catechol (phenolics), gramine (alkaloid) and L-ornithine-HCI (non-protein amino acid) on the activities of xenobiotic metabolizing enzymes in English grain aphid, Sitobion avenae, was evaluated. Phase I enzymes investigated in this study included carboxylesterase (CarE), and oxidoreductase, whereas Phase II enzymes were represented by glutathione S-transferase (GST). In general, CarE and GST activities in S. avenae were positively correlated with the concentration of plant secondary compounds in artificial diets. Oxidoreductase activity, however, displayed a different profile. Specifically, peroxidase (POD) and polyphenol oxidase (PPO) activities in S. avenae were positively correlated with concentrations of dietary catechol and gramine, respectively, whereas catalase (CAT) activity was significantly suppressed by the higher concentration of catechol, gramine and L-ornithine-HCl. These combined results suggest that CarE and GST in S. avenae are key enzymes to breakdown a broad spectrum of plant secondary compounds, whereas oxidoreductase, including PPO and POD, degrades specific groups of plant secondary compounds.