Bio-guided identification of proteins for the diagnosis of cysticercosis in swine
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- 作者：Priscilla Nativela ; priscilla.nativel@hotmail.fr" class="auth_mail" title="E-mail the corresponding author ; Anjanirina Rahantamalalaa ; anjanirina@pasteur.mg" class="auth_mail" title="E-mail the corresponding author ; Sitraka Ramiandrisoaa ; sixcool@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Virginie Rasoamampianinaaa ; virginie.rasoamampianina@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Magalie Duchateaub ; magalie.duchateau@pasteur.fr" class="auth_mail" title="E-mail the corresponding author ; Julia Chamot-Rookeb ; julia.chamot-rooke@pasteur.fr" class="auth_mail" title="E-mail the corresponding author ; Remy Guebeya ; rguebey@yahoo.fr" class="auth_mail" title="E-mail the corresponding author ; Harentsoaniaina Rasamoelina-Andriamanivoc ; harena23@yahoo.fr" class="auth_mail" title="E-mail the corresponding author ; Ronan Jamboua ; ronan.jambou@pasteur.fr" class="auth_mail" title="E-mail the corresponding author ; rjambou@pasteur.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Madagascar ; Taenia solium ; Cysticercosis ; 2D electrophoresis ; LC/MS-MS
- 刊名：Veterinary Parasitology
- 出版年：2016
- 出版时间：15 April 2016
- 年：2016
- 卷：220
- 期：Complete
- 页码：23-27
- 全文大小：876 K
文摘
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Diagnostic of cysticercosis must be drawn to gate set for animals, to sustain control strategy.
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Bioguided identification of proteins from the liquid of the cyst was done using serums from positive and negative animals.
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Exchange chromatography and 2D-electrophoresis was used to identify protein candidates to develop a diagnostic test.
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Mass spectroscopy allowed to identify 18 new proteins and 9 were selected for further development