Robust generation and expansion of skeletal muscle progenitors and myocytes from human pluripotent stem cells
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- 作者：Michael Sheltona ; Avetik Kocharyana ; b ; Jun Liua ; Ilona S. Skerjanca ; iskerjan@uottawa.ca" class="auth_mail" title="E-mail the corresponding author ; William L. Stanforda ; b ; c ; d ; wstanford@ohri.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：hESCs ; human embryonic stem cells ; iPSCs ; induced pluripotent stem cells ; PSCs ; pluripotent stem cells ; EBs ; embryoid bodies ; SMPs ; skeletal muscle progenitors ; TC ; tissue culture ; MRFs ; myogenic regulatory factors ; FACS ; fluorescence activated cell sorting ; BSA ; bovine serum albumin ; EGF ; epidermal growth factor ; FGF2 ; fibroblast growth factor 2 ; E8 ; Essential 8 media ; RPE ; retinal pigmented epithelial ; EMT ; epithelial to mesenchymal transition ; T ; Brachyury ; MSGN1 ; Mesogenin 1 ; GOI ; gene of in
- 刊名：Methods
- 出版年：2016
- 出版时间：15 May 2016
- 年：2016
- 卷：101
- 期：Complete
- 页码：73-84
- 全文大小：3057 K
文摘
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High concentrations of GSK3 inhibitor CHIR99021 induce paraxial mesoderm.
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Mixed populations of PAX7-positive cells and skeletal myocytes appear in 6 weeks.
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Routine passage of differentiating cells allows 1:3 expansion every 2 weeks.
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Differentiated myogenic populations can be cultured for upwards of 100 days.