Morgana/CHP-1 is a novel chaperone able to protect cells from stress

详细信息    查看全文

• 作者：Wojciech Michowski ; Roberta Ferretti ; Marta B. Wisniewska ; Mateusz Ambrozkiewicz ; Malgorzata Beresewicz ; Federica Fusella ; Anna Skibinska-Kijek ; Barbara Zablocka ; Mara Brancaccio ; Guido Tarone ; Jacek
• 关键词：Morgana/CHP-1 ; CHORD ; Co-chaperone ; Heat shock protein ; HSP90 ; stress
• 刊名：Biochimica et Biophysica Acta (BBA)/Molecular Cell Research
• 出版年：2010
• 出版时间：September 2010
• 年：2010
• 卷：1803
• 期：9
• 页码：1043-1049
• 全文大小：682 K

文摘

Morgana/CHP-1 (CHORD containing protein-1) has been recently shown to be necessary for proper cell divisions. However, the presence of the protein in postmitotic tissues such as brain and striated muscle suggests that morgana/CHP-1 has additional cellular functions. Here we show that morgana/CHP-1 behaves like an HSP90 co-chaperone and possesses an independent molecular chaperone activity towards denatured proteins. The expression time profile of morgana/Chp-1 in NIH3T3 cells in response to heat stress is similar to that of Hsp70, a classical effector of Heat Shock Factor-1 mediated stress response. Moreover, overexpression of morgana/CHP-1 in NIH3T3 cells leads to the increased stress resistance of the cells. Interestingly, morgana/Chp-1 upregulation in response to transient global brain ischemia lasts longer in ischemia-resistant regions of the gerbil hippocampus than in vulnerable ones, suggesting the involvement of morgana/CHP-1 in natural protective mechanisms in vivo.