The structure of the O-specific polysaccharide of the lipopolysaccharide from Yersinia enterocolitica serotype O:50 strain 3229

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• 作者：Agnieszka Becza?a ; Katarzyna Anna Duda ; Mikael Skurnik ; Otto Holst
• 关键词：Yersinia enterocolitica O ; 50 ; LPS structure ; Phage ¦µR1-37 ; NMR
• 刊名：Carbohydrate Research
• 出版年：2012
• 出版时间：1 October, 2012
• 年：2012
• 卷：359
• 期：Complete
• 页码：97-101
• 全文大小：391 K

文摘

The genus Yersinia represents Gram-negative bacteria that are widely distributed in the environment and possess lipopolysaccharide (LPS) as a major molecule on the surface of the bacterial envelope. LPS is composed of an anchoring lipid, termed lipid A, and a polysaccharide part which not only determines the bacterial serotype but may also serve as a bacteriophage receptor. Here we present the structure of the O-polysaccharide (O-PS) of Yersinia enterocolitica O:50 phage ¦µR1-37-sensitive strain 3229. The structural characterization was performed utilizing 1D and 2D nuclear magnetic resonance spectroscopy and chemical analyses. The structure of the O-PS biological repeating unit was identified as

¡ú2)-¦Á-l-Rhap-(1¡ú3)-¦Á-l-FucpNAc-(1¡ú3)-¦Á-l-FucpNAc-(1¡ú3)-¦Â-d-GlcpNAc-(1¡ú.