Endothelin-1 induced MCAO: Dose dependency of cerebral blood flow

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• 作者：Simona Nikolova ; Slavianka Moyanova ; Sarah Hughes ; Mir ; a Bellyou-Camilleri ; Ting-Yim Lee ; Robert Bartha
• 关键词：Cerebral blood flow ; Endothelin ; Ischemia ; Isoflurane ; Histology ; Stroke model ; Rat
• 刊名：Journal of Neuroscience Methods
• 出版年：2009
• 出版时间：30 April 2009
• 年：2009
• 卷：179
• 期：1
• 页码：22-28
• 全文大小：1154 K

文摘

The purpose of this study was to characterize the magnitude and duration of cerebral blood flow (CBF) reduction in the somatosensory cortical region in a rat model of middle cerebral artery occlusion (MCAO) induced by endothelin-1 (ET1) microinjection under isoflurane anesthesia. MCAO was induced by microinjection of ET1 proximal to the MCA in 41 isoflurane-anesthetized male Sprague–Dawley rats. Three doses of ET1 were studied, 60 pmol (Group 1), 150 pmol (Group 2), and 300 pmol (Group 3). CBF was monitored for 4 h following injection using a laser Doppler probe stereotaxically inserted into the left somatosensory cortical region. Computed tomography perfusion imaging was used to verify the extent and duration of blood flow reduction in a subset of 12 animals. The magnitude and duration of blood flow reduction was variable (60–92 % of baseline). The 300 pmol dose provided the greatest sustained decrease in blood flow. Evidence of tissue damage was obtained in cases where CBF decreased to <40 % of baseline. At the doses studied, ET1-induced ischemia in the presence of isoflurane anesthesia can be used as a minimally invasive but variable model of MCAO. The model is well suited for acute imaging studies of ischemia.