An injectable thiol-acrylate poly(ethylene glycol) hydrogel for sustained release of methylprednisolone sodium succinate

详细信息    查看全文

• 作者：Christopher D. Pritchard ; Timothy M. O’ ; Shea ; Daniel J. Siegwart ; Eliezer Calo ; Daniel G. Anderson ; Francis M. Reynolds ; John A. Thomas ; Jonathan R. Slotkin ; Eric J. Woodard ; Robert Langer
• 关键词：Cell adhesion ; Controlled drug release ; Hydrogel ; Peptide ; Polyethylene oxide ; Spinal surgery
• 刊名：Biomaterials
• 出版年：2011
• 出版时间：January 2011
• 年：2011
• 卷：32
• 期：2
• 页码：587-597
• 全文大小：1173 K

文摘

Clinically available injectable hydrogels face technical challenges associated with swelling after injection and toxicity from unreacted constituents that impede their performance as surgical biomaterials. To overcome these challenges, we developed a system where chemical gelation was controlled by a conjugate Michael addition between thiol and acrylate in aqueous media, with 97 % monomer conversion and 6 wt. % sol fraction. The hydrogel exhibited syneresis on equilibration, reducing to 59.7 % of its initial volume. It had mechanical properties similar to soft human tissue with an elastic modulus of 189.8 kPa. Furthermore, a mesh size of 6.9 nm resulted in sustained release of methylprednisolone sodium succinate with a loading efficiency of 2 mg/mL. Functionalization with 50 μg/mL of an oligolysine peptide resulted in attachment of freshly isolated murine mesenchymal stem cells. The rational design of the physical, chemical and biological properties of the hydrogel makes it a potentially promising candidate for injectable applications.