We develop an LC–MS/MS method measuring epigenetic modifications from cells.
HDAC increases histone lysine acetylation correlating with cell viability by MTS.
The effects on histone methylation and KDM expression depend on the HDAC inhibitor.
Mocetinostat increases histone lysine methylation and decreases expression of KDM.
Mocetinostat is both an HDAC inhibitor and a de facto KDM inhibitor.