Oxylipin biosynthesis in spikemoss Selaginella moellendorffii: Molecular cloning and identification of divinyl ether synthases CYP74M1 and CYP74M3
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- 作者：Svetlana S. Gorina ; Yana Y. Toporkova ; Lucia S. Mukhtarova ; Elena O. Smirnova ; Ivan R. Chechetkin ; Bulat I. Khairutdinov ; Yuri V. Gogolev ; Alexander N. Grechkin ; grechkin@kibb.knc.ru" class="auth_mail" title="E-mail the corresponding author
- 关键词：DES ; divinyl ether synthase ; HPL ; hydroperoxide lyase ; AOS ; allene oxide synthase ; EAS ; epoxyalcohol synthase ; 13(S)-HPOT ; (9Z ; 11E ; 13S ; 15Z)-13-hydroperoxyoctadecatrienoic acid ; 13(S)-HPOD ; (9Z ; 11E ; 13S)-13-hydroperoxyoctadecadienoic acid ; 9(S)-HPOD ; (9S ; 10E ; 12Z)-9-hydroperoxyoctadecadienoic acid ; IMAC ; immobilized metal affinity chromatography ; TMS ; trimethylsilyl ; GC&ndash ; MS ; gas chromatography&ndash ; mass spectrometry ; ECL ; equivalent chain lengths ; ORF ; open reading frame ; IHCD ; I-helix centra
- 刊名：Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：1861
- 期：4
- 页码：301-309
- 全文大小：1608 K
文摘
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Genes CYP74M1 and CYP74M3 of Selaginella moellendorffii were expressed in E. coli.
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Recombinant CYP74M1 and CYP74M3 exhibit the 13-divinyl ether synthase activity.
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CYP74M1 synthesizes (11Z)-etheroleic or (11Z)-etherolenic acids.
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CYP74M3 synthesizes mainly etheroleic or etherolenic acids.
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CYP74M1 and CYP74M3 are the first DESs detected in non-flowering plants.